Gt(ROSA)26Sor^{tm1.1(birA)Mejr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(birA)Mejr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Dies Meijer
• MGI ID: MGI:3588507
• Synonyms: bira, Rosa26^BirA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(birA)Mejr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100227
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: An HA (hemagglutinin) tagged E. coli bacterial birA, bifunctional protein (biotin ligase), gene was inserted into exon 3 of the rabbit beta-globin gene. The entire birA ORF, with the rabbit beta globin splice acceptor and transcription termination sequences were excised to generate a targeting vector that also contained a floxed PGK- puromycin selection cassette. This targeting vector was inserted into the first intron of the Gt(ROSA)26Sor gene. Cre-mediated recombination removed the floxed puromycin cassette. Western blot of mutants demonstrated the expression of birA in all tissues tested, though levels of expression varied from tissue to tissue. (J:100227)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 10 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:100227 Driegen S, et al., A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic Res. 2005 Aug;14(4):477-82
• All: 32 reference(s)