Tg(Gnrh1-Fgfr1)1Tsai
Transgene Detail

Summary

• Symbol: Tg(Gnrh1-Fgfr1)1Tsai
• Name: transgene insertion 1, Pei-San Tsai
• MGI ID: MGI:3588566
• Synonyms: dnFGFR, G-FGFRm-1
• Transgene: Tg(Gnrh1-Fgfr1)1Tsai Location: unknown
• Alliance: Tg(Gnrh1-Fgfr1)1Tsai page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gnrh1-Fgfr1)1Tsai expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fgfr1 (MGI:95522)

• Mutation details: A truncated form of Fgfr1 lacking the intracellular tyrosine kinase domain was ligated downstream of the rabbit beta-globin intron and placed under the control of the rat Gnrh1 promoter/enhancer region. The human growth hormone polyA sequence was inserted at the 3' end of the Fgfr1 cDNA. A transcript of approximately 1.5 kb was present only in the forebrain, where Gnhr1 neurons reside, corresponding to the size of the mutant Fgfr1. Expression was not found in the hindbrain or liver. (J:95057)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Another founder line, G-FGFRm-5, was also established, but two different transgene derived transcripts were detected. Expression of these transcripts were also detected in hindbrain as well as in the forebrain. The G-FGFRm-5 line was not studied further.

References

• Original: J:95057 Tsai PS, et al., Targeted expression of a dominant-negative fibroblast growth factor (FGF) receptor in gonadotropin-releasing hormone (GnRH) neurons reduces FGF responsiveness and the size of GnRH neuronal population. Mol Endocrinol. 2005 Jan;19(1):225-36
• All: 3 reference(s)