Dicer1^tm1Bdh
Targeted Allele Detail

Summary

• Symbol: Dicer1^tm1Bdh
• Name: dicer 1, ribonuclease type III; targeted mutation 1, Brian D Harfe
• MGI ID: MGI:3589208
• Synonyms: Dcr^flox, Dcr^fx, Dicer1^fl, dicer^fl, Dicer^flox, Dicer^lox
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^tm1Bdh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100475
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert loxP sites around the exon that encodes most of the second RNaseIII domain as well as an frt-flanked neo within the loxP-encompassed sequence. Cre-mediated removal of the sequence would result in the loss of 90 amino acids, a loss that does not create a downstream frame-shift or an unstable protein. (J:100475)

Disease models

Expression

In Mice Carrying this Mutation:	401 assay results 22 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	63 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Dicer1^tm1Bdh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 96 strains or lines available

References

• Original: J:100475 Harfe BD, et al., The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10898-903
• All: 237 reference(s)