Dicer1^tm1Dli
Targeted Allele Detail

Summary

• Symbol: Dicer1^tm1Dli
• Name: dicer 1, ribonuclease type III; targeted mutation 1, David M Livingston
• MGI ID: MGI:3589287
• Synonyms: dcr^fl
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^tm1Dli page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96031
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were inserted to flank exons 18-20, which encode part of the first RNase III domain. Deletion of this sequence putatively leads to out-of-frame splicing of all downstream exons, including the second RNase III and the dsRNA-binding domains, and results in complete ablation of enzymactic activity. An frt-flanked neo was inserted into intron 17 and was removed by crossing mice with a strain that expresses Flp recombinase in the germ line. (J:100546)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 96 strains or lines available

References

• Original: J:96031 Kanellopoulou C, et al., Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. Genes Dev. 2005 Feb 15;19(4):489-501
• All: 5 reference(s)