Ndst1^tm1.1Grob
Targeted Allele Detail

Summary

• Symbol: Ndst1^tm1.1Grob
• Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1; targeted mutation 1, Kay Grobe
• MGI ID: MGI:3589383
• Gene: Ndst1 Location: Chr18:60817566-60881722 bp, - strand Genetic Position: Chr18, 34.38 cM, cytoband D2
• Alliance: Ndst1^tm1.1Grob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100425
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cre mediated excision was used to remove exon 2, containing most of the 5' untranslated region and 171 of 882 amino acids of the open reading frame, containing the signal peptide, cytoplasmic tail, transmembrane region and part of the catalytic domain. Western blot of mutant embryo extract demonstrated lack of protein. In situ hybridization of E11.5 embryos failed to detect reactivity using an antisense riboprobe. Immunohistochemistry also confirmed successful protein ablation. (J:100425)

Expression

In Structures Affected by this Mutation:

18 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ndst1 Mutation: 47 strains or lines available

References

• Original: J:100425 Grobe K, et al., Cerebral hypoplasia and craniofacial defects in mice lacking heparan sulfate Ndst1 gene function. Development. 2005 Aug;132(16):3777-86
• All: 6 reference(s)