Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell
Transgene Detail

Summary

• Symbol: Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell
• Name: transgene insertion 1, John F Elliott
• MGI ID: MGI:3590082
• Synonyms: DQ8⁺, human CD4, DQ8 transgene, human CD4, DQA1*0301, DQB1*0302 transgene, Tg(CD2-CD4,HLA-DQA1*0301,HLA-DQB1*0302)1Ell, Tg(CD4,HLA-DQ8)1Ell
• Transgene: Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell Location: unknown
• Alliance: Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell page

Transgene origin

• Strain of Origin: NOD/MrkTac

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell expresses 3 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CD4 (920)

  Cd4	(MGI:88335)

  human

  HLA-DQA1 (3117)

  H2-Aa

  (MGI:95895)

  human

  HLA-DQB1 (3119)

  H2-Ab1

  (MGI:103070)

• Mutation details: Fertilized oocytes were co-injected with two DNA fragments: a human CD4 cDNA joined to the promoter from the human CD2 gene and a large DNA fragment, reassembled from segments of the cosmid pDCbeta2, containing the human major histocompatibility (MHC) class II alleles HLA-DQA1*0301 and HLA-DQB*0302, which together encode the dimer comprising the HLA-DQ8 molecule, plus 5' regulatory elements. Human CD4 is expressed at a low level on all T cells of transgenic mice. HLA-DQ8 is expressed in homozygous NOD mice at a level comparable to that of the endogenous NOD H2-Abeta^g7 MHC class II molecule in wild-type NOD mice. (J:87013, J:113267)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Although in NOD mice homozygous for this transgene alone endogeous NOD H2-Abeta^g7 and transgene-derived HLA-DQ8 are expressed at similar levels, virtually all CD4⁺ T-cell responses are H2Abeta1^g7 restricted (J:87013).

References

• Original: J:87013 Liu J, et al., Major DQ8-restricted T-cell epitopes for human GAD65 mapped using human CD4, DQA1*0301, DQB1*0302 transgenic IA(null) NOD mice. Diabetes. 1999 Mar;48(3):469-77
• All: 4 reference(s)