Rr149356^tm1.1Pf
Targeted Allele Detail

Summary

• Symbol: Rr149356^tm1.1Pf
• Name: regulatory region 149356; targeted mutation 1.1, Pierre Ferrier
• MGI ID: MGI:3590151
• Synonyms: DeltaEbeta^-, Ebeta^-, Tcrbe^tm1Pf
• Gene: Rr149356 Location: Chr6:41526801-41532817 bp, . strand Genetic Position: Chr6, Syntenic
• Alliance: Rr149356^tm1.1Pf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34311
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: The 560-bp HpaINcoI restriction fragment containing Tcrb enhancer Ebeta was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent cre-mediated recombination. Mutants were shown to lack Tcrb rearrangement by DNA-PCR and RNA-PCR analyses although germ-line Vbeta transcripts were detectable at a low level. (J:34311)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr149356 Mutation: 0 strains or lines available

References

• Original: J:34311 Bouvier G, et al., Deletion of the mouse T-cell receptor beta gene enhancer blocks alphabeta T-cell development. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7877-81
• All: 15 reference(s)