Spred2^Gt(XB228)Byg
Gene trapped Allele Detail

Summary

• Symbol: Spred2^Gt(XB228)Byg
• Name: sprouty-related EVH1 domain containing 2; gene trap XB228, BayGenomics
• MGI ID: MGI:3590299
• Synonyms: Spred-2^-, Spred2^{Gt(pGTO)1Ksch}
• Gene: Spred2 Location: Chr11:19874442-19974026 bp, + strand Genetic Position: Chr11, 12.79 cM, cytoband A3.2
• Alliance: Spred2^Gt(XB228)Byg page

Achondroplasia-like dwarfism in Spred2^Gt(XB228)Byg/Spred2^Gt(XB228)Byg mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: XB228
• Germline Transmission: Earliest citation of germline transmission: J:100826
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: A gene trap vector, pGTO, containing a splice acceptor, Betageo and SV40 pA sequence, inserted into intron 4 of the locus. RT-PCR failed to detect transcript in all tissues tested. Northern blot and Western blot confirmed lack of all forms of transcript and protein in mutant brain samples, respectively. (J:100826)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB228	CC144423 ; (MGI Seq Detail)	5' RACE	Chr11:19948166-19958475 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  222 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	368 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spred2 Mutation: 222 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:100826 Bundschu K, et al., Gene disruption of Spred-2 causes dwarfism. J Biol Chem. 2005 Aug 5;280(31):28572-80
• All: 8 reference(s)