Gad1^tm1.1Tama
Targeted Allele Detail

Summary

• Symbol: Gad1^tm1.1Tama
• Name: glutamate decarboxylase 1; targeted mutation 1.1, Nobuaki Tamamaki
• MGI ID: MGI:3590301
• Synonyms: Gad67-Gfp, Gad67^Gfp, Gad67::gfp, GAD67-GFP (delta neo), GAD-GFP
• Gene: Gad1 Location: Chr2:70392109-70432358 bp, + strand Genetic Position: Chr2, 41.63 cM, cytoband D
• Alliance: Gad1^tm1.1Tama page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86389
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Crossing Gad1^tm1Tama with mice expressing cre recombinase in the germline removed the loxP-flanked neo. Western blot demonstrated that the cerebral cortex contained more GFP than the cerebral cortex of animals with the neo present. Fluorescent microscopy confirmed the same findings in mutant brain samples. (J:86389)

Expression

In Mice Carrying this Mutation:

292 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gad1 Mutation: 65 strains or lines available

References

• Original: J:86389 Tamamaki N, et al., Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse. J Comp Neurol. 2003 Dec 1;467(1):60-79
• All: 289 reference(s)