Rhotm2Jlem
Targeted Allele Detail
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Symbol: |
Rhotm2Jlem |
Name: |
rhodopsin; targeted mutation 2, Janis Lem |
MGI ID: |
MGI:3590580 |
Synonyms: |
Palm- |
Gene: |
Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
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Alliance: |
Rhotm2Jlem page
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Germline Transmission: |
Earliest citation of germline transmission:
J:100845
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Parent Cell Line: |
J1 (ES Cell)
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Strain of Origin: |
129S4/SvJae
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: A targeting construct was designed to insert Cys322Thr and Cys323Ser modifications in the locus to eliminate both palmitoylation sites. A tACE-Cre-neo cassette was included in the construct and self-excised upon passage through the male germ line. Expression of palmitoylation-deficient rhodopsin transcript was demonstrated by RT-PCR, and the absence of palmitates on rhodopsin protein of mutant mice was confirmed by mass spectrometry.
(J:100845)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Rho Mutation: |
51 strains or lines available
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Original: |
J:100845 Wang Z, et al., Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation-deficient rhodopsin. J Biol Chem. 2005 Jul 1;280(26):24293-300 |
All: |
4 reference(s) |
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