Rps6^tm1Om
Targeted Allele Detail

Summary

• Symbol: Rps6^tm1Om
• Name: ribosomal protein S6; targeted mutation 1, Oded Meyuhas
• MGI ID: MGI:3603166
• Synonyms: rpS6^KI, rpS6^P-
• Gene: Rps6 Location: Chr4:86772897-86775649 bp, - strand Genetic Position: Chr4, 40.69 cM, cytoband C3
• Alliance: Rps6^tm1Om page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100952
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Serine residues 235, 236, 240, 244 and 247 in exon 5 were replaced with alanines. A loxP-flanked neo was inserted into intron 5. RT-PCR analysis demonstrated expression of the mutant allele. Western blot failed to detect any phosphorylation of these sites in livers of untreated or partially hepatectomized mutant mice. Furthermore, metabolic labelling of mouse embryo fibroblasts indicates that no compensatory phosphorylation occurs on any other phosphorylatable residues. (J:100952)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rps6 Mutation: 12 strains or lines available

References

• Original: J:100952 Ruvinsky I, et al., Ribosomal protein S6 phosphorylation is a determinant of cell size and glucose homeostasis. Genes Dev. 2005 Sep 15;19(18):2199-211
• All: 11 reference(s)