Fhl2^tm2Wlm
Targeted Allele Detail

Summary

• Symbol: Fhl2^tm2Wlm
• Name: four and a half LIM domains 2; targeted mutation 2, R Sanders Williams
• MGI ID: MGI:3603305
• Synonyms: Fhl2^LacZ+, FLH2^-, FLH2^LacZ/neo
• Gene: Fhl2 Location: Chr1:43162234-43236144 bp, - strand Genetic Position: Chr1, 22.33 cM
• Alliance: Fhl2^tm2Wlm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100289
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector was designed to replace the coding sequences from exon 2, including the translation initiation codon, the N-terminal half LIM motif, part of the LIM1 domain, and 0.5 kb of intron 2 with a lacZ reporter gene and a loxP flanked neomycin resistance cassette. Transcription of the lacZ insertion was evident in various stages of embryonic development. Absence of endogenous transcript and protein in mutants was confirmed by Northern and Western blots, respectively. (J:100289)

Expression

In Mice Carrying this Mutation:	19 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fhl2 Mutation: 16 strains or lines available

References

• Original: J:100289 Kong Y, et al., Cardiac-specific LIM protein FHL2 modifies the hypertrophic response to beta-adrenergic stimulation. Circulation. 2001 Jun 5;103(22):2731-8
• All: 22 reference(s)