Tg(Ins2-GAD2)2Lt
Transgene Detail

Summary

• Symbol: Tg(Ins2-GAD2)2Lt
• Name: transgene insertion 2, Edward H Leiter
• MGI ID: MGI:3603349
• Synonyms: TgN(RIP7hGAD65)Lt4 A-line, TgN(RIP7hGAD65)Lt4 (A line)
• Transgene: Tg(Ins2-GAD2)2Lt Location: unknown Genetic Position: Chr15, Syntenic
• Alliance: Tg(Ins2-GAD2)2Lt page

Transgene origin

• Strain of Origin: NOD/ShiLt

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-GAD2)2Lt expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GAD2 (2572)

  Gad2	(MGI:95634)

• Tg(Ins2-GAD2)2Lt expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgene comprises the cDNA sequence encoding human glutamic acid decarboxylase 2 under control of a modified rat insulin 2 promoter composed of 9.5 kb of genomic DNA from upstream of the transcription initiation site fused to the first intron of Ins2. Densitrometric Southern blot analysis indicates presence of 4-5 copies of the transgene. FISH analysis and pseudo-Giemsa metaphase spread analysis showed integration to have occurred near the centromere of Chr 15. Expression in the pancreatic islets of transgenic mRNA has been demonstrated by reverse transcription followed by PCR (RT-PCR), and of GAD2 protein by western blot analysis. (J:100251)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:100251 Bridgett M, et al., Differential protection in two transgenic lines of NOD/Lt mice hyperexpressing the autoantigen GAD65 in pancreatic beta-cells. Diabetes. 1998 Dec;47(12):1848-56
• All: 1 reference(s)