Mib1^tm1Kong
Targeted Allele Detail

Summary

• Symbol: Mib1^tm1Kong
• Name: MIB E3 ubiquitin protein ligase 1; targeted mutation 1, Young-Yun Kong
• MGI ID: MGI:3603514
• Synonyms: Mib1^-, mib1-lacZ
• Gene: Mib1 Location: Chr18:10725548-10818704 bp, + strand Genetic Position: Chr18, 5.35 cM
• Alliance: Mib1^tm1Kong page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101173
• Parent Cell Line: E14K (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ-puro was inserted into exon 6, deleting exon 7 encoding amino acids 303-364. In situ hybridization demonstrated broad expression of lacZ throughout the body and abundantly in branchial arches, presumptive limb buds and somites of embryo. Northern blot demonstrated expression at various embryonic stages. (J:101173)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mib1 Mutation: 55 strains or lines available

References

• Original: J:101173 Koo BK, et al., Mind bomb 1 is essential for generating functional Notch ligands to activate Notch. Development. 2005 Aug;132(15):3459-70
• All: 4 reference(s)