Insig2^tm1Mbjg
Targeted Allele Detail

Summary

• Symbol: Insig2^tm1Mbjg
• Name: insulin induced gene 2; targeted mutation 1, Joseph L Goldstein
• MGI ID: MGI:3603525
• Synonyms: Insig-2-, Insig2^-, Insig2^tm1Jlg
• Gene: Insig2 Location: Chr1:121232082-121260318 bp, - strand Genetic Position: Chr1, 52.91 cM, cytoband E2
• Alliance: Insig2^tm1Mbjg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101203
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A polIIsneopA cassette was inserted to replace exons 2 and 3, which encode the first 123 of 225 amino acids. Northern blot and immunoblot failed to detect wild-type transcript and protein, respectively. An aberrant transcript was detected, possibly due to an initiation site in exons 1a or 1b and splicing directly to exon 4, 5 or 6. In such a transcript, the first available start codon is in exon 5, and, if translated, this transcript would yield a 23 amino-acid peptide. (J:101203)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Insig2 Mutation: 15 strains or lines available

References

• Original: J:101203 Engelking LJ, et al., Schoenheimer effect explained - feedback regulation of cholesterol synthesis in mice mediated by Insig proteins. J Clin Invest. 2005 Sep;115(9):2489-98
• All: 8 reference(s)