Htt^tm3Szi
Targeted Allele Detail

Summary

• Symbol: Htt^tm3Szi
• Name: huntingtin; targeted mutation 3, Scott Zeitlin
• MGI ID: MGI:3603606
• Synonyms: Hdh^deltaQ
• Gene: Htt Location: Chr5:34919084-35069878 bp, + strand Genetic Position: Chr5, 17.92 cM
• Alliance: Htt^tm3Szi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106763
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to delete the (CAG)2CAA(CAG)4 triplet repeat encoding 7 glutamines within exon 1. A FLAG epitope tag was included at the N-terminus of the coding sequence in exon 1 to follow expression. A floxed PGK-neo was inserted ~1.3 kb upstream of the transcriptional start site in the opposite transcription orientation.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Htt Mutation: 178 strains or lines available

References

• Original: J:106763 Clabough EB, et al., Deletion of the triplet repeat encoding polyglutamine within the mouse Huntington's disease gene results in subtle behavioral/motor phenotypes in vivo and elevated levels of ATP with cellular senescence in vitro. Hum Mol Genet. 2006 Feb 15;15(4):607-23
• All: 2 reference(s)