Srf^tm1Rjs
Targeted Allele Detail

Summary

• Symbol: Srf^tm1Rjs
• Name: serum response factor; targeted mutation 1, Robert J Schwartz
• MGI ID: MGI:3604097
• Synonyms: SRF^-, SRF^LacZ
• Gene: Srf Location: Chr17:46859255-46867101 bp, - strand Genetic Position: Chr17, 22.9 cM
• Alliance: Srf^tm1Rjs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101471
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Sequence encoding the DNA binding and dimerization domains of the protein was replaced by the insertion of an NLS-betagalactosidase-neomycin cassette. In situ hybridization demonstrated expression in embryos ranging from E7.5 to E9.5 predominantly in the cardiac crescent and later in the heart tube, the somites, and the precondensed mesenchyme in the tail, consistent with the expression of the endogenous gene. (J:101471)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Srf Mutation: 27 strains or lines available

References

• Original: J:101471 Barron MR, et al., Serum response factor, an enriched cardiac mesoderm obligatory factor, is a downstream gene target for Tbx genes. J Biol Chem. 2005 Mar 25;280(12):11816-28
• All: 4 reference(s)