Rassf1^tm1.2Brd
Targeted Allele Detail

Summary

• Symbol: Rassf1^tm1.2Brd
• Name: Ras association (RalGDS/AF-6) domain family member 1; targeted mutation 1.2, Allan Bradley
• MGI ID: MGI:3604205
• Synonyms: Rossf1A^Brdm2
• Gene: Rassf1 Location: Chr9:107428752-107439460 bp, + strand Genetic Position: Chr9, 58.12 cM
• Alliance: Rassf1^tm1.2Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101369
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Transfecting ES cells containing a floxed 1alpha exon with mice expressing cre recombinase resulted in the excision of the exon, thereby ablating isoform 1A expression. RT-PCR of lung confirmed absence of transcript from the exon, while the 1C isoform remained unaffected. (J:101369)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Rassf1^tm1.2Brd

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rassf1 Mutation: 26 strains or lines available

References

• Original: J:101369 van der Weyden L, et al., The RASSF1A isoform of RASSF1 promotes microtubule stability and suppresses tumorigenesis. Mol Cell Biol. 2005 Sep;25(18):8356-67
• All: 8 reference(s)