Map3k4^tm1Glj
Targeted Allele Detail

Summary

• Symbol: Map3k4^tm1Glj
• Name: mitogen-activated protein kinase kinase kinase 4; targeted mutation 1, Gary L Johnson
• MGI ID: MGI:3604209
• Synonyms: MEK4^K1361R, MEKK4^K1361R
• Gene: Map3k4 Location: Chr17:12446508-12537683 bp, - strand Genetic Position: Chr17, 8.42 cM
• Alliance: Map3k4^tm1Glj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101350
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Two mutations were inserted into exon 21: a silent mutation in codon 1357 from CTG to CTC, and an AAG to AGG in 1361 that produced the replacement of the active site lysine by an arginine. Western blot of mutant MEFs demonstrated the expression of protein was comparable to wild-type, and inactivation of the kinase activity was validated in vivo. (J:101350)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Map3k4 Mutation: 78 strains or lines available

References

• Original: J:101350 Abell AN, et al., Ablation of MEKK4 kinase activity causes neurulation and skeletal patterning defects in the mouse embryo. Mol Cell Biol. 2005 Oct;25(20):8948-59
• All: 5 reference(s)