Pag1^tm1Kplm
Targeted Allele Detail

Summary

• Symbol: Pag1^tm1Kplm
• Name: phosphoprotein associated with glycosphingolipid microdomains 1; targeted mutation 1, Kong-Peng Lam
• MGI ID: MGI:3604217
• Synonyms: Cbp^-
• Gene: Pag1 Location: Chr3:9752539-9898739 bp, - strand Genetic Position: Chr3, 2.47 cM
• Alliance: Pag1^tm1Kplm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101365
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The last coding exon was deleted, thereby by abolishing activity by removing the critical Tyr-314 residue and the C-terminal VTRL motif. Northern blot confirmed lack of full-length or truncated transcript in mutant cells. Absense of protein in Western blot supported this finding. (J:101365)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pag1 Mutation: 21 strains or lines available

References

• Original: J:101365 Xu S, et al., Cbp deficiency alters Csk localization in lipid rafts but does not affect T-cell development. Mol Cell Biol. 2005 Oct;25(19):8486-95
• All: 2 reference(s)