Polq^tm1Jow
Targeted Allele Detail

Summary

• Symbol: Polq^tm1Jow
• Name: polymerase (DNA directed), theta; targeted mutation 1, Jiyang O-Wang
• MGI ID: MGI:3604448
• Gene: Polq Location: Chr16:36832148-36915779 bp, + strand Genetic Position: Chr16, 26.32 cM, cytoband B4
• Alliance: Polq^tm1Jow page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101409
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A targeting vector was designed to replace exons 25 and 26 with a neomycin resistance gene. Removing this sequence inactivated the DNA polymerase activity but left the helicase and other potentially important domains intact. The deletion was in-frame and left the promoter intact, therefore the remaining locus could theoretically be transcribed and translated. RT-PCR proved this to be true, although the level of mutant transcript was slightly reduced, possibly due to the intronic insertion of the neo gene. Western blot analysis further confirmed presence of mutant protein at a slightly reduced level. (J:101409)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polq Mutation: 127 strains or lines available

References

• Original: J:101409 Masuda K, et al., DNA polymerase theta contributes to the generation of C/G mutations during somatic hypermutation of Ig genes. Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13986-91
• All: 1 reference(s)