Inpp5a^{Gt(OST50073)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Inpp5a^{Gt(OST50073)Lex}
• Name: inositol polyphosphate-5-phosphatase A; gene trap OST50073, Lexicon Genetics
• MGI ID: MGI:3604476
• Synonyms: Inpp5a^Gt694Lex
• Gene: Inpp5a Location: Chr7:138969025-139159568 bp, + strand Genetic Position: Chr7, 84.57 cM
• Alliance: Inpp5a^{Gt(OST50073)Lex} page

Mutation origin

• Mutant Cell Line: OST50073
• Germline Transmission: Earliest citation of germline transmission: J:103485
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector
• Mutation details: Retroviral gene trap insertion occurred between exons 8 and 9. The insertion truncated the protein after amino acid 215, removing half of the protein including the predicted active site. RT-PCR analysis of P0 mutants confirmed elimination of wild-type mRNA. No truncated protein was detected by Western blot analysis; however, a small amount of normal protein was detected in both homozygous and heterozygous mutants, probably due to a small amount of splicing around the gene trap. (J:103485, J:249381)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST50073	CG503351 ; (MGI Seq Detail)	3' RACE	Chr7:139118092-139147688 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Inpp5a Mutation: 34 strains or lines available

Notes

See also, data as provided by Lexicon Genetics, Inc.

References

• Original: J:103485 Lexicon Genetics Inc, NIH initiative supporting placement of Lexicon Genetics, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 3 reference(s)