Cck^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Cck^tm1Dgen
• Name: cholecystokinin; targeted mutation 1, Deltagen
• MGI ID: MGI:3604503
• Gene: Cck Location: Chr9:121318890-121324728 bp, - strand Genetic Position: Chr9, 72.43 cM, cytoband F4
• Alliance: Cck^tm1Dgen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101679
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the brain cortex, moderate levels in whole brain, subcortical region, cerebellum and olfactory bulb, and low levels in brainstem, spinal cord, eye, Harderian glands, heart, lung, liver, pancreas, kidney, spleen, thymus, lymph nodes, skin, gallbladder, urinary bladder, pituitary gland, adrenal gland, salivary gland, skeletal muscle, tongue, stomach, small intestine, cecum, colon, testis, epididymis, seminal vesicle, coagulating gland, prostate gland, ovary, uterus and white fat. However, beta-galactosidase expression from this allele is detectable in only in brain, kidney and testis, with moderate lacZ expression detected in some regions of the whole mount brain including areas of the cortex and hippocampus. On coronal sections of brain, very weak staining was observed in a few cells of the pyramidal cell layer in hippocampus. Strong, scattered expression was observed in renal medulla and papilla. Strong staining was also observed in a few spermatogenic cells in the testis.

Expression

In Mice Carrying this Mutation:

36 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cck Mutation: 26 strains or lines available

Notes

See also, data as provided by Deltagen, Inc.

References

• Original: J:101679 Deltagen Inc, NIH initiative supporting placement of Deltagen, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 1 reference(s)