| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutation: |
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Disruption caused by insertion of vector
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Mutation details: A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in skin, urinary bladder, tongue and uterus, moderate levels in eye, pancreas, thymus, gallbladder, stomach, epididymis, seminal vesicle, prostate gland, ovary and white fat, and low levels in olfactory bulb, Harderian glands, heart, lung, kidney, spleen, lymph nodes, adrenal gland, salivary gland, skeletal muscle, small intestine, large intestine, cecum, testis and coagulating gland. However, beta-galactosidase expression from this allele is detectable in only in epithelium in the urinary bladder, trachea and skin and in tracheal cartilage as well as in a few spermatogenic cells in the testis and stromal cells in the ovary. A small piece of tissue adjacent to the heart also showed staining.
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