Chrna2^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Chrna2^tm1Dgen
• Name: cholinergic receptor nicotinic alpha 2 subunit; targeted mutation 1, Deltagen
• MGI ID: MGI:3604548
• Gene: Chrna2 Location: Chr14:66372488-66390397 bp, + strand Genetic Position: Chr14, 34.36 cM
• Alliance: Chrna2^tm1Dgen page

Mutation origin

• Mutant Cell Line: 5414
• Germline Transmission: Earliest citation of germline transmission: J:101679
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. While RT-PCR analysis detects a gene transcript in all tissues tested, strong beta-galactosidase expression from this allele is detectable only in a small region of the whole mount brainstem. No expression was detected on coronal sections of brain. Moderate staining was observed in the ovarian stroma.

Expression

In Mice Carrying this Mutation:

36 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna2 Mutation: 33 strains or lines available

Notes

See also, data as provided by Deltagen, Inc.

References

• Original: J:101679 Deltagen Inc, NIH initiative supporting placement of Deltagen, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 3 reference(s)