Sema5a^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Sema5a^tm1Dgen
• Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; targeted mutation 1, Deltagen
• MGI ID: MGI:3604576
• Gene: Sema5a Location: Chr15:32244959-32696487 bp, + strand Genetic Position: Chr15, 13.02 cM
• Alliance: Sema5a^tm1Dgen page

Mutation origin

• Mutant Cell Line: 5115
• Germline Transmission: Earliest citation of germline transmission: J:101679
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. While RT-PCR analysis detects a gene transcript in many of the tissues analyzed, highest levels are detected in ovary, and beta-galactosidase expression from this allele is detectable in almost all tissues and organs examined.

Expression

In Mice Carrying this Mutation:

114 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sema5a Mutation: 67 strains or lines available

Notes

See also, data as provided by Deltagen, Inc.

References

• Original: J:101679 Deltagen Inc, NIH initiative supporting placement of Deltagen, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 3 reference(s)