Spnw1^DBA/2
QTL Variant Detail

Summary

• QTL variant: Spnw1^DBA/2
• Name: spleen weight 1; DBA/2
• MGI ID: MGI:3605123
• QTL: Spnw1 Location: unknown Genetic Position: Chr11, Syntenic

Variant origin

• Strain of Specimen: DBA/2

Variant description

• Allele Type: QTL

Notes

Spnw1 interacts with Spnw2, Spnw3, and Spnw4. Animals homozygous for DU6i-derived alleles at Spnw4 and homozygous for DBA/2-derived alleles at Spnw1 exhibit increased spleen weights.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:101006

Linkage analysis was performed on 411 (DU6i x DBA/2)F2 intercross animals to identify QTL associated with body weight and composition traits. 93 microsatellite markers at an average spacing of 14.1 cM were used for the genome scan. Parental strain DU6i is extremely fat and exhibits increased body size compared to parental strain DBA/2. Animals were phenotyped at 42 days of age. Several interacting locus pairs affecting abdominal fat percentage, abdominal fat weight, body weight, kidney weight, and spleenweight were detected. Locus pairs exceeding the 5% genome-wide significance level are reported in this summary.

Linkage to abdominal fat percentage, Abfp1 (abdominal fat percentage 1), mapped to 34 cM on mouse Chromosome 17. This locus interacts with Abfp2 (abdominal fat percentage 2) at 58 cM on mouse Chromosome 11 and Abfp3 (abdominal fat percentage 3) at 16 cM on mouse Chromosome 8. Abfp4 (abdominal fat percentage 4) maps to 26 cM on mouse Chromosome 3 and interacts with Abfp5 (abdominal fat percentage 5) at 20 cM on mouse Chromosome 5. Animals heterozygous for DBA/2- and DU6i-derived alleles at Abpf4 and homozygous for DU6i-derived alleles at Abfp5 exhibit increased abdominal fat percentages.

Linkage to abdominal fat weight mapped to 60 cM on mouseChromosome 11 and is named Abfw1 (abdominal fat weight 1). This locus interacts with Abfw2 (abdominal fat weight 2) at 64 cM on mouse Chromosome 4, Abfw3 (abdominal fat weight 3) at 32 cM on mouse Chromosome 17, and Abfw4 (abdominal fat weight 4) at 43 cM on mouse Chromosome 19. Abfw5 (abdominal fat weight 5) mapped to 72 cM on mouse Chromosome 5 and interacts with Abfw6 (abdominal fat weight 6) at 6 cM on mouse Chromosome 12.

Linkage to body weight, Bodw1 (body weight 1), mapped to 18 cM on mouse Chromosome 2. This locus interacts with Bodw2 (body weight 2) at 55 cM on mouse Chromosome 11. Animals homozygous for DU6i-derived alleles at both Bodw1 and Bodw2 exhibit increased body weights. Bodw3 (body weight 3) maps to 5 cM on mouse Chromosome 1 and thislocus interacts with Bodw4 (body weight 4) at 34 cM on mouse Chromosome 9. Animals heterozygous for DU6i- and DBA/2-derived alleles at Bodw3 and homozygous for DBA/2-derived alleles at Bodw4 exhibit decreased body weights. The reciprocal genotype (homozygous for DU6i-derived alleles at Bodw3 and heterozygous at Bodw4) also confers decreased body weight.

Three interacting locus pairs linked to kidney weight were identified. Knyw1 (kidney weight 1) mapped to 0 cM on mouse Chromosome 12 and Knyw2 (kidney weight 2) mapped to 24 cM on mouse Chromosome 15. Knyw3 (kidney weight 3) mapped to 38 cM on mouse Chromosome 2 and Knyw4 (kidney weight 4) mapped to 25 cM on mouse Chromosome 9. Animals homozygous for DU6i-derived alleles at Knyw3 and Knyw4 exhibit increased kidney weights. Knyw5 (kidney weight 5) and Knyw6 (kidney weight 6) mapped to mouse Chromosome 1 at 35 cM and 80 cM, respectively.

Linkage to spleen weight mapped to 37 cM on mouse Chromosome 11. This locus is named Spnw1 (spleen weight 1) and shows interaction with several other loci. Spnw1 interacts with Spnw2 (spleen weight 2) at 63 cM on mouse Chromosome 3, Spnw3 (spleen weight 3) at 70 cM on mouse Chromosome 1, and Spnw4 (spleen weight 4) at 19 cM on mouse Chromosome 16. Animals homozygous for DU6i-derived alleles at Spnw4 and homozygous for DBA/2-derived alleles at Spnw1 exhibit increased spleen weights. Spnw3 also interacts with a locus at 21 cM on mouse Chromosome 2 named Spnw5 (spleen weight 5). Lastly, Spnw6 (spleen weight 6) at 50 cM on mouse Chromosome 2 shows interaction with Spnw7 (spleen weight 7) at 5 cM on mouse Chromosome 12.

References

• Original: J:101006 Carlborg O, et al., Simultaneous mapping of epistatic QTL in DU6i x DBA/2 mice. Mamm Genome. 2005 Jul;16(7):481-94
• All: 1 reference(s)