Tg(Scgb1a1-Scnn1b)6608Bouc
Transgene Detail

Summary

• Symbol: Tg(Scgb1a1-Scnn1b)6608Bouc
• Name: transgene insertion 6608, Richard Boucher
• MGI ID: MGI:3605471
• Synonyms: betaENaC-Tg
• Transgene: Tg(Scgb1a1-Scnn1b)6608Bouc Location: unknown Genetic Position: Chr5, Syntenic
• Alliance: Tg(Scgb1a1-Scnn1b)6608Bouc page

Transgene origin

• Strain of Origin: (C3H x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Scgb1a1-Scnn1b)6608Bouc expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Scnn1b (MGI:104696)

• Mutation details: Approximately 2330 base pairs of 5' flanking sequence from the rat airway-specific Scgb1a1 (also called the Clara cell secretory protein) gene were inserted into the pTG1 vector upstream of exon 1 (104 base pairs, including the transcription initiation site), intron 1 (947 base pairs) and 10 base pairs of exon 2 from the mouse transthyretin gene; the protein-coding region of the mouse Scnn1b cDNA, inserted just downstream of the transthyretin exon 2 splice acceptor, is followed by the SV40 early-region polyadenylation signal. In situ hybridization with a transgene-specific antisense probe confirmed expression in airway epithelia of the lung. The insertion site was identified by in situ hybridization; it is located on chromosome 5 near the B-C1 chromosome band junction. (J:91139, J:101822, J:101823, J:220663)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available

Notes

A second transgenic line, Tg(Scgb1a1-Scnn1b)6047Bouc, was created in the same genetic background. The phenotypes of the two lines are indistinguishable.

Complete histological examination of multiple organs and systems reveals no pathology of organs other than lungs of transgenic mice.

References

• Original: J:91139 Mall M, et al., Increased airway epithelial Na+ absorption produces cystic fibrosis-like lung disease in mice. Nat Med. 2004 May;10(5):487-93
• All: 45 reference(s)