Tg(Lim2*G15V)1Rlc
Transgene Detail

Summary

• Symbol: Tg(Lim2*G15V)1Rlc
• Name: transgene insertion 1, Robert L Church
• MGI ID: MGI:3606782
• Synonyms: Lim2^To3 transgene
• Transgene: Tg(Lim2*G15V)1Rlc Location: unknown
• Alliance: Tg(Lim2*G15V)1Rlc page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Lim2*G15V)1Rlc expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Lim2 (MGI:104698)

• Mutation details: The transgene comprises 6.3 kb of genomic DNA containing the entire mouse Lim2 gene, including 399 bp of 5' flanking DNA, with the G-to-T transversion in exon 2 putatively responsible for the dominant total opacity 3 (To3) phenotype. Dot-blot and Southern blot analyses of genomic DNA from transgenic mice indicate presence of two co-integrated copies. RT-PCR of total mouse lens RNA followed by digestion of the PCR product with BstE II, which cuts within the mutant, but not the wildtype, Lim2 cDNA, revealed a low but reproducible ratio of transgenic to wildtype mRNA in lenses from fetal (E18), weanling (P~20) and adult (P>20) transgenic mice. (J:100078)
• Inheritance: Semidominant

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Only one founder, 26011F, transmitted the transgene and the eye phenotype; a second transgenic mouse, also having the eye phenotype, failed to transmit either. Absolute concordance of transgene dosage and the presence and severity of the eye phenotype in >100 progeny of founder 26011F and her offspring verified that the Lim2 mutation identified in To3 mice is responsible for the dominantly inherited To3 phenotype.

References

• Original: J:100078 Steele EC Jr, et al., Lim2(To3) transgenic mice establish a causative relationship between the mutation identified in the lim2 gene and cataractogenesis in the To3 mouse mutant. Mol Vis. 2000 Jun 2;6:85-94
• All: 1 reference(s)