Oc90^df1H5R
Radiation induced Allele Detail

Summary

• Symbol: Oc90^df1H5R
• Name: otoconin 90; df1H5R
• MGI ID: MGI:3606974
• Gene: Oc90 Location: Chr15:65747902-65784246 bp, - strand Genetic Position: Chr15, 29.16 cM
• Alliance: Oc90^df1H5R page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102039
• Parent Cell Line: EC7.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129X1/SvJ)F1

Mutation description

• Allele Type: Radiation induced (Not Applicable)
• Mutations: Deletion, Insertion
• Oc90^df1H5R involves 1 genes/genome features (Oc90) View all
• Mutation details: An HSV-tk/beta-actin-neo cassette was inserted into intron 2 using a gene targeting strategy. ES cells expressing both markers were selected. After X-irradiation of properly targeted ES cell lines with 400 rad, loss of tk expression was determined using FIAV. Cells no longer expressing tk were further tested for loss of neomycin expression. SSLP typing of polymorphic microsatellite markers determined that this allele contains a deletion of greater than 148 kb and may be a skipping deletion. FISH confirmed deletion of genomic sequence. (J:102039)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oc90 Mutation: 26 strains or lines available

References

• Original: J:102039 Chick WS, et al., X-ray-induced deletion complexes in embryonic stem cells on mouse chromosome 15. Mamm Genome. 2005 Sep;16(9):661-71
• All: 1 reference(s)