Spag16^tm1Jfs
Targeted Allele Detail

Summary

• Symbol: Spag16^tm1Jfs
• Name: sperm associated antigen 16; targeted mutation 1, Jerome F Strauss III
• MGI ID: MGI:3606977
• Gene: Spag16 Location: Chr1:69866129-70764291 bp, + strand Genetic Position: Chr1, 35.04 cM, cytoband C3
• Alliance: Spag16^tm1Jfs page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:92446
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exon 11 with a PGK-neo selection cassette, preventing expression of the 35-kDA protein and the WD repeats of the 71-kDa protein. (J:92446)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spag16 Mutation: 66 strains or lines available

Notes

Haploinsufficiency of Spag16 prevents genetic transmission of the mutated allele and generation of non-chimeric heterozygous mice. All phenotype data reported are from chimeric mice.

References

• Original: J:92446 Zhang Z, et al., Haploinsufficiency for the murine orthologue of Chlamydomonas PF20 disrupts spermatogenesis. Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12946-51
• All: 3 reference(s)