Spag16^tm2Jfs
Targeted Allele Detail

Summary

• Symbol: Spag16^tm2Jfs
• Name: sperm associated antigen 16; targeted mutation 2, Jerome F Strauss III
• MGI ID: MGI:3606979
• Synonyms: Spag16L null, Spag16_pr1^-
• Gene: Spag16 Location: Chr1:69866129-70764291 bp, + strand Genetic Position: Chr1, 35.04 cM, cytoband C3
• Alliance: Spag16^tm2Jfs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107006
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to replace exons 2 and 3 with a neomycin resistance cassette, thereby preventing expression of the 71-kDa protein, but allowing expression of the 35-kDa protein. Northern and immunoblot analyses confirmed absence of wild type transcript and protein in mutant. A truncated 0.5 kb mRNA was detected in mutant mice, a truncated protein corresponding to the long isoform was not detected. (J:107006)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spag16 Mutation: 66 strains or lines available

References

• Original: J:107006 Zhang Z, et al., Deficiency of SPAG16L Causes Male Infertility Associated with Impaired Sperm Motility. Biol Reprod. 2006 Apr;74(4):751-9
• All: 8 reference(s)