Clip1^tm1Gal
Targeted Allele Detail

Summary

• Symbol: Clip1^tm1Gal
• Name: CAP-GLY domain containing linker protein 1; targeted mutation 1, Niels Galjart
• MGI ID: MGI:3607145
• Synonyms: CLIP-170 KO
• Gene: Clip1 Location: Chr5:123715857-123822527 bp, - strand Genetic Position: Chr5, 63.06 cM
• Alliance: Clip1^tm1Gal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102107
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A GFP-loxP-pMC1neo-loxP cassette was inserted in front of the ATG in exon 2. The neo is in antisense orientation. Northern blot demonstrated the lack of mRNA in brain, heart, skeletal muscle, MEFs and testis; however, transcript was detected in lung and embryonic extracts, indicating the allele is a hypomorph. The testis specific isoform was not affected by the targeting event, and was expressed in mutants. (J:102107)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clip1 Mutation: 83 strains or lines available

References

• Original: J:102107 Akhmanova A, et al., The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis. Genes Dev. 2005 Oct 15;19(20):2501-15
• All: 3 reference(s)