Mib1^tm1Art
Targeted Allele Detail

Summary

• Symbol: Mib1^tm1Art
• Name: MIB E3 ubiquitin protein ligase 1; targeted mutation 1, Karen Artzt
• MGI ID: MGI:3607998
• Gene: Mib1 Location: Chr18:10725548-10818704 bp, + strand Genetic Position: Chr18, 5.35 cM
• Alliance: Mib1^tm1Art page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102528
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting event deleted a part of exon 1 containing the translation initiation codon, replacing it with a DNA segment containing a lacZ gene and a Pgk-neo cassette. The bacterial beta-galactosidase is not expressed in vivo. RT-PCR analysis of homozygous mutant mice amplified no transcript, and immunoblot analysis detected only cross-reacting proteins and not the major, specific band. (J:102528)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	49 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mib1 Mutation: 55 strains or lines available

References

• Original: J:102528 Barsi JC, et al., Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech Dev. 2005 Oct;122(10):1106-17
• All: 1 reference(s)