Anth2^CBA/J
QTL Variant Detail

Summary

• QTL variant: Anth2^CBA/J
• Name: resistance to Bacillus anthracis 2; CBA/J
• MGI ID: MGI:3609202
• QTL: Anth2 Location: unknown Genetic Position: Chr2, Syntenic

Variant origin

• Strain of Specimen: CBA/J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers resistance to the Sterne strain of Bacillus anthracis compared to A/J. (J:9501)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:9501

Anth2 is a previously identified QTL for resistance/susceptibility to infection with the Sterne strain of Bacillus anthracis. Anth2 is located on mouse Chromosome 2 and shows association with the Hc locus at 23.5 cM. Hc appears to be an attractive candidate gene for Anth2 as resistant inbred mouse strains CBA/J and BALB/cJ carry functional Hc genes while the susceptible inbred mouse strain A/J carries deficient Hc genes. The following experiments support the candidacy of Hc as the gene responsible for Anth2.

The association of Sterne resistance/susceptibility was evaluated in 46 (A/J x CBA/J)F1 x A/J backcross animals and 48 (A/J x CBA/J)F1 x CBA/J backcross animal. None of the (A/J x CBA/J)F1 x CBA/J backcross animals died after infection with 10⁵ units of Sterne B.anthracis while 39% of (A/J x CBA/J)F1 x A/J backcross animals succumbed to infection. All of the animals that died displayed ^{1% Hc activity while all surviving animals displayed between 11%-100% Hc activity. (%Hc activity was measured relative to the Hc activity in resistant parental strain CBA/J.) Interestingly, surviving backcross animals with pre-infection Hc activity levels between 15%-33% showed an increase to 68%-95% post-infection.}

Sterne resistance/susceptibility was also evaluated in a population of 46 (A/J x CBA/J)F2 animals. Only 5 (11%) F2 animals died after infection with 10<5 units of Sterne B.anthracis. Again, all of the F2 animals that died from Sterne infection were negative for Hc activity while 98% of the surviving F2 animals were positive for Hc activity.

A group of 7 different AXL (A=A/J; L=C57L/J) recombinant inbred (RI) strain and 7 different BXD (B=C57BL/6J; D=DBA/J) RI strains were infected with 10⁵ units of Sterne B.anthracis and evaluated for survival. 70%-100% of animals from Hc negative RI strains died after Sterne infection compared to 1%-14% of animals from Hc positive RI strains.

References

• Original: J:9501 Welkos SL, et al., Pathogenesis and genetic control of resistance to the Sterne strain of Bacillus anthracis. Microb Pathog. 1988 Jan;4(1):53-69
• All: 1 reference(s)