Prkce^tm1Bsca
Targeted Allele Detail

Summary

• Symbol: Prkce^tm1Bsca
• Name: protein kinase C, epsilon; targeted mutation 1, Lisardo Bosca
• MGI ID: MGI:3609215
• Synonyms: PKCepsilon^-
• Gene: Prkce Location: Chr17:86475213-86965347 bp, + strand Genetic Position: Chr17, 56.74 cM
• Alliance: Prkce^tm1Bsca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103337
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert stop codons in all three reading frames, an IRES-lacZ-pA and a neomycin resistance gene into exon 1. Northern and Western blot analyses confirmed that transcript and protein were absent in mutants. (J:103337)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkce Mutation: 40 strains or lines available

References

• Original: J:103337 Castrillo A, et al., Protein kinase Cepsilon is required for macrophage activation and defense against bacterial infection. J Exp Med. 2001 Nov 5;194(9):1231-42
• All: 6 reference(s)