Pbwg18^{M. m. castaneus}
QTL Variant Detail

Summary

• QTL variant: Pbwg18^{M. m. castaneus}
• Name: postnatal body weight growth 18; M. m. castaneus
• MGI ID: MGI:3609414
• QTL: Pbwg18 Location: Chr14:69281984-69282125 bp Genetic Position: Chr14, cM position of peak correlated region/allele: 35.98 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: M. m. castaneus

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: This allele confers decreased weight gain between 6-10 weeks of age in female animals compared to C57BL/6J. (J:102250)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:102250

Linkage analysis was performed on 387 (C57BL/6J x M. m. castaneus)F1 x M. m. castaneus backcross animals to identify QTL associated with postnatal body weight and growth traits. 90 polymorphic markers were used for the genome scan and average intermarker distance varied between 19.5 cM - 20.2 cM. Animals were weighed weekly between 3 - 10 weeks of age. Parental strain M. m. castaneus exhibits 60% smaller body size compared to parental strain C57BL/6J.

Eight previously mapped growth QTL (Pbwg1-Pbwg7, Pbwg9) were detected and re-confirmed in this study. Novel QTL Pbwg14-Pbwg22 were mapped in this experiment. Several of these loci participate in epistatic interactions. M. m. castaneus-derived alleles confer decreased body weight and/or weight gain at allloci except Pbwg9 and Pbwg19 where M. m. castaneus-derived alleles confer increased body weight and/or weight gain.

Pbwg1 mapped to 33 cM on mouse Chromosome 2 with marker D2Mit324 (LOD=3.1-10.9) and is associated with body weight at 4-10 weeks of age, weight gain from 3-6 weeks of age, and weight gain from 6-10 weeks of age. The minimal 95% confidence interval of Pbwg1 spans 17 cM.

Pbwg2 mapped to 62 cM on mouse Chromosome 4 near D4Mit32 (LOD=3.1) and is associated with body weight at 9 weeks of age. The 95%confidence interval of Pbwg2 spans 46 cM.

Female-specific QTL Pbwg14 mapped to 1 cM on mouse Chromosome 5 with marker D5Mit345 (LOD=2.8) and is associated with body weight at 8 weeks of age. The 95% confidence interval of Pbwg14 spans 76 cM.

Pbwg3 mapped to 72 cM on mouse Chromosome 7 with marker D7Mit259 (LOD=2.6-3.6) and is associated with body weight at 5-9 weeks of age. The minimal 95% confidence interval of Pbwg3 spans 56 cM.

Pbwg15 and Pbwg4 mapped to mouse Chromosome 9 at 43 cM and 69 cM, respectively. Pbwg15 peaks near D9Mit340 (LOD=2.6-2.7) and is associated with body weight at 3-4 weeks of age. Pbwg4 peaks near D9Mit18 (LOD=3.5) and is associated with body weight at 5 weeks of age. The minimal 95% confidence intervals for Pbwg4 and Pbwg15 span 62cM.

Pbwg5 mapped to 68 cM on mouse Chromosome 10 near D10Mit145 (LOD=2.6) and is associated with body weight at 7 weeks of age. The 95% confidence interval of Pbwg5 spans 76 cM. Pbwg9 and Pbwg16 mapped to 14 cM (LOD=3.5 at D10Mit16) and 45cM (LOD=4.6 at D10Mit20), repectively. Pbwg9 is associated with weight gain from 3-6 weeks of age and Pbwg16 is associated with body weight at 3 weeks of age. Unlike the other loci, M. m. castaneus-derived alleles at Pbwg9 confer increased weight gain.

Pbwg6 and Pbwg17 mapped to 53 cM (LOD=4.2-5.1 at D13Mit195) and 46 cM (LOD=4.2-4.9 at D13Mit195) on mouse Chromosome 13, respectively. Pbwg17 is associated with body weight at 6-10 weeks of age and Pbwg6 is associated with body weight at 5-10 weeks of age.The minimal95% confidence intervals of Pbwg17 and Pbwg6 spans 35 cM and 40 cM, respectively.

Female-specific QTL Pbwg18 mapped to 30 cM on mouse Chromosome 14 with marker D14Mit159 (LOD=4.3). The 95% confidence interval of Pbwg18 spans 42 cM and this locus is associated with weight gain from 6 to 10 weeks of age.

Male-specific QTL Pbwg19 mapped to 2 cM on mouse Chromosome 16 with marker D16Mit32 (LOD=3). The 95% confidence interval of Pbwg19 spans 69 cM and this locus is associated with body weight at5 weeks of age. Unlike the other QTL, M. m. castaneus-derived alleles at Pbwg19 confer increased body weight in males.

Pbwg20 mapped to 0 cM on mouse Chromosome 19 with marker D19Mit29 (LOD=3.1-6.3) and is associated with body weight at 3-4 weeks of age. The minimal 95% confidence intervalofPbwg20 spans 25 cM.

Male-specific QTL Pbwg7 and Pbwg21 mapped to mouse Chromosome X at 17 cM and 27 cM, repectively. Pbwg7 is associated with body weight at 5-8 weeks of age and weight gain between 3-6 weeks of age(LOD=3.4-3.8 at DXMit75). The minimum 95% confidence interval of Pbwg7 spans 44 cM. Pbwg21 is associated with body weight at 3 weeks of age and weight gain between 3-6 weeks of age (LOD=2.6-3.6 at DXMit75).

Female-specific QTL Pbwg22 mapped to 35 cM onmouse Chromosome X near DXMit19 (LOD=2.9-4.3). The minimal 95% confidence interval of Pbwg22 spans 39 cM and this locus is associated with body weight at 9-10 weeks of age.

References

• Original: J:102250 Ishikawa A, et al., Further mapping of quantitative trait loci for postnatal growth in an inter-sub-specific backcross of wild Mus musculus castaneus and C57BL/6J mice. Genet Res. 2005 Apr;85(2):127-37
• All: 1 reference(s)