Nr1i2^tm1Sakl
Targeted Allele Detail

Summary

• Symbol: Nr1i2^tm1Sakl
• Name: nuclear receptor subfamily 1, group I, member 2; targeted mutation 1, Steven A Kliewer
• MGI ID: MGI:3609633
• Synonyms: PXR^-, Pxr^{tm1Glaxo-Wellcome}
• Gene: Nr1i2 Location: Chr16:38068711-38115211 bp, - strand Genetic Position: Chr16, 26.64 cM
• Alliance: Nr1i2^tm1Sakl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103505
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene replaced the first coding exon, which includes the first zinc finger of the DNA-binding domain, via homologous recombination. Northern blot and RT-PCR detected transcript ~200 bp shorter than the wild-type transcript, as expected. Sequence analysis revealed that exon 1 was fused to exon 3 in the mutant animal. The truncated transcript was present at reduced levels. If this RNA is translated from a spurious initiation site, the protein is expected to be nonfunctional. (J:103505)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Nr1i2 Mutation: 35 strains or lines available

References

• Original: J:103505 Staudinger JL, et al., The nuclear receptor PXR is a lithocholic acid sensor that protects against liver toxicity. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3369-74
• All: 41 reference(s)