Nt5e^tm1Jsc
Targeted Allele Detail

Summary

• Symbol: Nt5e^tm1Jsc
• Name: 5' nucleotidase, ecto; targeted mutation 1, Jurgen Schrader
• MGI ID: MGI:3609975
• Synonyms: Cd73^-
• Gene: Nt5e Location: Chr9:88209662-88254142 bp, + strand Genetic Position: Chr9, 47.24 cM, cytoband E3.2
• Alliance: Nt5e^tm1Jsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102299
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The floxed exon 2 and neomycin resistance gene were deleted from the Nt5e^tm2Jsc allele via cre-mediated excision. RT-PCR detected a shortened transcript at levels similar to wild-type. The mutation caused a frame shift at the new exon 1 to 3 junction, resulting in the termination of translation after the first exon. Protein was not detected in Western blot. (J:102299)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nt5e Mutation: 30 strains or lines available

References

• Original: J:102299 Koszalka P, et al., Targeted disruption of cd73/ecto-5'-nucleotidase alters thromboregulation and augments vascular inflammatory response. Circ Res. 2004 Oct 15;95(8):814-21
• All: 17 reference(s)