Otx2^{tm1(Dkk1)Imat}
Targeted Allele Detail

Summary

• Symbol: Otx2^{tm1(Dkk1)Imat}
• Name: orthodenticle homeobox 2; targeted mutation 1, Isao Matsuo
• MGI ID: MGI:3609983
• Synonyms: Otx2^ddk
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^{tm1(Dkk1)Imat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103272
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: The locus was disrupted by the insertion of Dkk1 cDNA and the PGK-neo cassette flanked by two loxP sites. The neomycin cassette was removed by crossing with mice expressing cre recombinase in the germ line. Dkk1 expression was detected in epiblast at E6.5 and in the entire rostral brain region at E7.8, in addition to endogenous expression domains. RT-PCR confirmed the absence of Otx2 transcript in mutant embryos. (J:103272)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:103272 Kimura-Yoshida C, et al., Canonical Wnt signaling and its antagonist regulate anterior-posterior axis polarization by guiding cell migration in mouse visceral endoderm. Dev Cell. 2005 Nov;9(5):639-50
• All: 1 reference(s)