Tcra^tm1Slek
Targeted Allele Detail

Summary

• Symbol: Tcra^tm1Slek
• Name: T cell receptor alpha chain; targeted mutation 1, Barry P Sleckman
• MGI ID: MGI:3610385
• Synonyms: TCRalpha/delta^I
• Gene: Tcra Location: Chr14:52665424-54461655 bp, + strand Genetic Position: Chr14, cytoband D1-D2

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101719
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Inversion
• Tcra^tm1Slek involves 61 genes/genome features (Traj61, Traj60, Traj59 ...) View all
• Mutation details: Cre recombinase was used to invert a 75-kb loxP-flanked region of the locus. The region encompasses all of the J-alpha gene segments, the C-alpha gene segment and enhancer E-alpha. In this allele the J-alpha gene segments lie in the opposite transcriptional orientation compared to the V-alpha gene segments. Accordingly, initial V-alpha to J-alpha rearrangements on the allele occur by inversion, resulting in retention of the Tcrd genes that lie within the locus in the mature alpha-beta T cells. (J:101719)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcra Mutation: 101 strains or lines available

References

• Original: J:101719 Khor B, et al., Chromosomal excision of TCRdelta chain genes is dispensable for alphabeta T cell lineage commitment. Int Immunol. 2005 Mar;17(3):225-32
• All: 1 reference(s)