Prkaca^tm3Gsm
Targeted Allele Detail

Summary

• Symbol: Prkaca^tm3Gsm
• Name: protein kinase, cAMP dependent, catalytic, alpha; targeted mutation 3, G Stanley McKnight
• MGI ID: MGI:3610390
• Synonyms: CalphaR
• Gene: Prkaca Location: Chr8:84699622-84723072 bp, + strand Genetic Position: Chr8, 40.22 cM, cytoband C3
• Alliance: Prkaca^tm3Gsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103418
• Parent Cell Line: REK2 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A W196R mutation was introduced into exon 7 by homolgous recombination. A loxP flanked neomycin resistance gene containing the SV40 polyA sequence was inserted into intron 5. Western blot demonstrated that mutant cells express half of the level of protein as that of wild-type cells. Cre-mediated recombination restores the expression of the mutant transcript containing the point mutation, and expression of the mutant protein. (J:103418)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkaca Mutation: 22 strains or lines available

References

• Original: J:103418 Niswender CM, et al., Cre recombinase-dependent expression of a constitutively active mutant allele of the catalytic subunit of protein kinase A. Genesis. 2005 Nov;43(3):109-19
• All: 8 reference(s)