Afp^{tm1(Cebpa)Ndw}
Targeted Allele Detail

Summary

• Symbol: Afp^{tm1(Cebpa)Ndw}
• Name: alpha fetoprotein; targeted mutation 1, Nai-dy Wang
• MGI ID: MGI:3610508
• Gene: Afp Location: Chr5:90638596-90656766 bp, + strand Genetic Position: Chr5, 44.77 cM
• Alliance: Afp^{tm1(Cebpa)Ndw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103404
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: Cebpa cDNA and a neomycin resistance gene in opposite orientation replaced exon 1 of the alpha fetoprotein locus. Quantitative RT-PCR of fetal livers demonstrated that mutants had a higher level of Cebpa mRNA than wild-type livers. It was undetermined whether those higher levels were due to expression of the knock-in gene alone or due to a combination of the knock-in gene plus elevated expression of the endogenous gene. (J:103404)

Expression

In Mice Carrying this Mutation:

40 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Afp Mutation: 32 strains or lines available

References

• Original: J:103404 Tan EH, et al., CCAAT/enhancer binding protein alpha knock-in mice exhibit early liver glycogen storage and reduced susceptibility to hepatocellular carcinoma. Cancer Res. 2005 Nov 15;65(22):10330-7
• All: 1 reference(s)