Pik3c2b^tm1.1Pkha
Targeted Allele Detail

Summary

• Symbol: Pik3c2b^tm1.1Pkha
• Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta; targeted mutation 1.1, Paul A Khavari
• MGI ID: MGI:3611683
• Synonyms: C2beta^-
• Gene: Pik3c2b Location: Chr1:132973410-133036429 bp, + strand Genetic Position: Chr1, 57.75 cM
• Alliance: Pik3c2b^tm1.1Pkha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103754
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cre mediated recombination resulted in the deletion of exons 3-5. Loss of this sequence produced a truncated, nonfunctional protein lacking the PIK, catalytic, and C2 domains that are required for induction of keratinocyte differentiation in vitro. Transcript was not detected in keratinocytes from mutants. Western blot failed to detect protein in mutant epidermis as well as brain and testis. (J:103754)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3c2b Mutation: 82 strains or lines available

References

• Original: J:103754 Harada K, et al., The class II phosphoinositide 3-kinase C2beta is not essential for epidermal differentiation. Mol Cell Biol. 2005 Dec;25(24):11122-30
• All: 4 reference(s)