Kcnk3^tm1Sgb
Targeted Allele Detail

Summary

• Symbol: Kcnk3^tm1Sgb
• Name: potassium channel, subfamily K, member 3; targeted mutation 1, Stephen G Brickley
• MGI ID: MGI:3611768
• Synonyms: TASK-1 ^-
• Gene: Kcnk3 Location: Chr5:30745514-30782614 bp, + strand Genetic Position: Chr5, 16.68 cM, cytoband B
• Alliance: Kcnk3^tm1Sgb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103747
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, encoding the first transmembrane domain through the beginning of the first P1 loop was disrupted by homologous recombination. An IRES-lacZ-SV40pA-neo cassette containing a stop codon in all three reading frames replaced the first 21 amino acids. In situ hybridization and real time PCR showed that the targeted allele is not expressed in either mutant brain or heart samples. (J:103747)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnk3 Mutation: 34 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Primary aldosteronism in homozygous mice (J:188335)

References

• Original: J:103747 Aller MI, et al., Modifying the subunit composition of TASK channels alters the modulation of a leak conductance in cerebellar granule neurons. J Neurosci. 2005 Dec 7;25(49):11455-67
• All: 18 reference(s)