Car4^tm1Sly
Targeted Allele Detail

Summary

• Symbol: Car4^tm1Sly
• Name: carbonic anhydrase 4; targeted mutation 1, William S Sly
• MGI ID: MGI:3611770
• Synonyms: CAIV^dl1Sws, CA IV KO
• Gene: Car4 Location: Chr11:84848612-84856870 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Car4^tm1Sly page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103742
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl^Sl-J/J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The self-excising tACE-CRE-neo cassette replaced exons 1b, 2, 3, and 9 amino acids of exon 4. This sequence encodes all three active site His residues in the 146 amino acids of the protein that immediately follow the signal sequence. The resulting frameshift produced an early termination in exon 4. Absence of transcript was confirmed by RT-PCR of mutant brain, heart, kidney and muscle. Western blot analyses of the same tissues did not detect protein. (J:103742)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Car4 Mutation: 25 strains or lines available

Notes

ES cell line = W9.5s

References

• Original: J:103742 Shah GN, et al., Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16771-6
• All: 11 reference(s)