Car14^tm1Sly
Targeted Allele Detail

Summary

• Symbol: Car14^tm1Sly
• Name: carbonic anhydrase 14; targeted mutation 1, William S Sly
• MGI ID: MGI:3611771
• Synonyms: CAXIV^dl1Sws, CA XIV KO
• Gene: Car14 Location: Chr3:95805080-95812003 bp, - strand Genetic Position: Chr3, 41.48 cM
• Alliance: Car14^tm1Sly page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103742
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl^Sl-J/J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was used to replace exon 4, 16 bp of intron 3, and 13 bp of intron 4 with the self-excising tACE-CRE-neo cassette. The frameshift resulting from recombination putatively results in early termination in exon 5. RT-PCR failed to detect the predicted 0.75 fragment in brain, heart, kidney, liver or muscle samples. Protein was not detected in Western blot analysis of the same tissues. (J:103742)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Car14 Mutation: 19 strains or lines available

Notes

ES cell line = W9.5s

References

• Original: J:103742 Shah GN, et al., Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16771-6
• All: 8 reference(s)