Gabra3^tm2Uru
Targeted Allele Detail

Summary

• Symbol: Gabra3^tm2Uru
• Name: gamma-aminobutyric acid type A receptor subunit alpha 3; targeted mutation 2, Uwe Rudolph
• MGI ID: MGI:3611843
• Synonyms: alpha3 KO
• Gene: Gabra3 Location: ChrX:71476287-71700454 bp, - strand Genetic Position: ChrX, 37.24 cM
• Alliance: Gabra3^tm2Uru page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103831
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion vector IV-3KO was used to insert a cassette containing an FRT-flanked neo with a 3' loxP site and a copy of exon 4 with a second loxP site downstream. The duplication of exon 4 was predicted to shift the reading frame and prevent formation of the protein. Crossing with mice expressing FLP recombinase removed the neomycin resistance gene. RT-PCR failed to detect transcript containing the wild-type allele or the duplicate exon. Northern blot, Western blot and immunohistochemical analyses confirmed absence of transcript and protein in mutants, respectively. (J:103831)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabra3 Mutation: 8 strains or lines available

References

• Original: J:103831 Yee BK, et al., A schizophrenia-related sensorimotor deficit links alpha 3-containing GABAA receptors to a dopamine hyperfunction. Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17154-9
• All: 13 reference(s)