Crebbp^tm2Pkb
Targeted Allele Detail

Summary

• Symbol: Crebbp^tm2Pkb
• Name: CREB binding protein; targeted mutation 2, Paul K Brindle
• MGI ID: MGI:3612048
• Synonyms: CBP^deltaCH1
• Gene: Crebbp Location: Chr16:3899198-4031864 bp, - strand Genetic Position: Chr16, 2.4 cM
• Alliance: Crebbp^tm2Pkb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103607
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Portions of the CH1 domain (amino acids 342-393) was deleted and a neomycin resistance cassette flanked by loxp sites was introduced to the upstream intron. Transient infection of the ES cells with a Cre-expressing vector excised the neomycin cassette. RT-PCR confirmed the deletion in mutant MEFs. (J:103607)

Disease models

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Crebbp Mutation: 100 strains or lines available

References

• Original: J:103607 Kasper LH, et al., Two transactivation mechanisms cooperate for the bulk of HIF-1-responsive gene expression. EMBO J. 2005 Nov 16;24(22):3846-58
• All: 4 reference(s)