Ep300^tm3Pkb
Targeted Allele Detail

Summary

• Symbol: Ep300^tm3Pkb
• Name: E1A binding protein p300; targeted mutation 3, Paul K Brindle
• MGI ID: MGI:3612049
• Synonyms: p300^deltaCH1
• Gene: Ep300 Location: Chr15:81470329-81536273 bp, + strand Genetic Position: Chr15, 38.16 cM
• Alliance: Ep300^tm3Pkb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103607
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Intragenic deletion
• Mutation details: Portions of the CH1 domain (amino acids 329-379) encoded by exon 4 were deleted and a neomycin resistance cassette flanked by FRT sites was introduced to the downstream intron. Transient infection of the ES cells with a Flp-expressing vector excised the neomycin cassette. RT-PCR confirmed the deletion in mutant MEFs. (J:103607)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ep300 Mutation: 98 strains or lines available

References

• Original: J:103607 Kasper LH, et al., Two transactivation mechanisms cooperate for the bulk of HIF-1-responsive gene expression. EMBO J. 2005 Nov 16;24(22):3846-58
• All: 2 reference(s)